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MPL W515L/K has been shown to promote tumorigenesis in vivo [5], and a higher mutant allele frequency is associated with progression to myelofibrosis [17]. Thus, detecting MPL W515L/K mutations and determining the rel...
MPL gene regions covering the S505N and W515L/K mutations were subjected to PCR amplification and bidirectional sequencing in duplicate to identify sequence variations. This assay has a sensitivity to detect approxima...
The MPL W515L mutation occurs in the MPL amphipathic domain that serves as a hinge between the transmembrane and cytoplasmic domains. Expression of this mutation in 32D, UT7, and Ba/F3 cells demonstrated that it is ac...
The ipsogen MPL W515L/K Muta Screen Kit is a ready-to-use kit for the detection and qualitative analysis of the MPL W515L and W515K mutations independently in human genomic DNA (see figure "MPL mutations").
Ruan G-R, Jiang B, Li L-D, Niu J-H, Li J-L, Xie M et al (2009) MPL W515L/K mutations in 343 Chinese adults with JAK2V617F mutation-negative chronic myeloproliferative disorders detected by a newly developed.
SCIENTIFIC BACKGROUND Myeloproliferative neoplasms (MPNs) are hematological malignancies characterized by the proliferation of one or more myeloid lineages: granulocytic, erythroid, megakaryocytic and/or mast cell. Th...
Nov 15, 2006 · Recently, a gain-of-function MPL mutation, MPL W515L, was described in patients with JAK2 V617F-negative myelofibrosis with myeloid metaplasia (MMM). To gain more information on mutational frequency, di...
Methods and findings: DNA sequence analysis of the exons encoding the transmembrane and juxtamembrane domains of EPOR, MPL, and GCSFR, and comparison with germline DNA derived from buccal swabs, identified a somatic a...
Feb 5, 2009 · We conclude that MPL W515L occurs in a considerable proportion of acute megakaryoblastic leukaemias with myelofibrosis unrelated to PMF.
common (such as p.W515L) and alternate (such as p.W515A) vari-ants, increases the diagnostic yield. The development and valida-tion of the MPL LDT allows for clinically sensitive detection (5% for mutations in codon 5...
